1498 In vitro study of the impact of diabetic patient cells on cutaneous wound healing and angiogenesis

Diabetic ulcers (DU), common pathology for diabetic patients, is a chronic wound which struggle to heal and may require amputation of the lower legs. We propose replicate complete human skin an endothelialized dermal tissue in laboratory using cells extracted from patients treatment development. Populations fibroblasts keratinocytes were isolated patient harvested after foot amputation. The dWHM obtained by seeding these population healthy endothelial on chitosan-collagen sponges culturing them at air/liquid interface. Fibroblasts endothelials are seeded second sponge. Once epidermal layer mature, performed with biopsy punch sponge containing keratinocytes. reepithelialization then monitored 8 days. For ERCT, HUVEC transfected express GFP luciferase fibroblasts. number estimated through fluorescence produce addition luciferin ATP lysate. Controls produced same way age-matched subject populations In 2D culture, have reduce proliferation capacity (respectively 32,33% 44,56% less divisions per day, p<0,05). WHM, able form mature but organized epidermis than control had slower closure, day 2 WHM 32,63 ± 4,470 % (p<0,05) closed compared models. fibroblast 62,95% VEGF lead 55,10% capillaries formation. successfully healing model recreate condition ulcer, suitable development new treatments DU.